Trans Fat Analysis by NMR

A series of Trans Fat standards was purchased from AOCS. The ability of 1H and 13C NMR to predict Trans Fat Content as well as 

Saturated, Poly-unsaturated, and Mono-unsaturated Fat Content

The data of the samples is presented in the table below:

 

 

PLS regression techniques were used to correlate 1H and 13C NMR spectral variation to the unsaturation level and type of unsaturation of the samples.

 

Processed 13C data is shown below:

 

 

1H NMR data is shown below:

 

 

The following correlations were obtained from the 13C NMR data.